galnt7 rabbit polyclonal  (Novus Biologicals)

Journal: Cancer Research Communications

Article Title: GALNT7 Stratifies dMMR/MSI Colorectal Cancer into Distinct Molecular Subsets Associated with Prognosis and PD-L1 Expression

doi: 10.1158/2767-9764.CRC-25-0270

Figure Lengend Snippet: Integration of bulk, cell line, and single-cell RNA-seq for 188 glycosyltransferase genes identified five genes that were differentially expressed between MSI and MSS colorectal cancers. A, Heatmap depicting the upregulation of GALNT7 , GALNT1 , and HPSE and the downregulation of GALNT6 and ST6GAL1 in MSI colorectal cancer bulk tissues (TCGA cohort), cell lines (CCLE cohort), and single epithelial cancer cells (SMC cohort), compared with those of MSS. Dot size corresponds to the percentage of cells expressing the gene. B, Heatmap depicting the performance of Glyco-MSI score based on the expression of five genes to distinguish MSI from MSS colorectal cancers using 19 transcriptomic and proteomic cohorts of colorectal cancer. C, Forest plot demonstrating Cox HRs and 95% CIs of each five gene and the Glyco-MSI score for OS in TCGA cohort, PFS in the AC-ICAM cohort, and RFS in the GSE39582 cohort. D–F, Kaplan–Meier curves according to the expression of GALNT7 in TCGA ( D ), AC-ICAM ( E ), and GSE39582 ( F ). Log-rank P values, Cox HRs, and 95% CIs are indicated. For survival analysis, patients were dichotomized as high or low based on the median expression of GALNT7 .

Article Snippet: Endogenous peroxidases were blocked and antigens retrieved, and the slides were incubated overnight at 4°C with primary rabbit polyclonal anti-GALNT7 antibody (Atlas Antibodies Cat. #HPA064243, RRID: AB_2685223; 1:500).

Techniques: RNA Sequencing, Expressing

Journal: Cancer Research Communications

Article Title: GALNT7 Stratifies dMMR/MSI Colorectal Cancer into Distinct Molecular Subsets Associated with Prognosis and PD-L1 Expression

doi: 10.1158/2767-9764.CRC-25-0270

Figure Lengend Snippet: Prognostic impact of GALNT7 and the association between GALNT7 and PD-L1 ( CD274 ) in colorectal cancer, stratified by MSI status. A–F, Kaplan–Meier curves for OS in TCGA cohort ( A and D ), PFS in the AC-ICAM cohort ( B and E ), and RFS in the GSE39582 cohort ( C and F ), analyzed separately for MSI ( A–C ) and MSS ( D–F ) colorectal cancers according to the expression of GALNT7 . Patients were dichotomized as high or low based on the median expression of GALNT7 in each analysis. Log-rank P values, Cox HRs, and 95% CIs are indicated. G–I, Heatmaps depicting MSI status, GALNT7 expression, CMS, BRAF mutations, TMB, immune infiltration estimated using the ESTIMATE, and the expression of immune checkpoint genes in TCGA ( G ), AC-ICAM ( H ), and GSE39582 ( I ). J–L, Correlations between CD274 and GALNT7 mRNA expression according to MSI status in TCGA ( J ), AC-ICAM ( K ), and GSE39582 ( L ). M, Correlations between CD274 and GALNT7 protein expression according to MSI status in CPTAC. Pearson correlation coefficients are indicated. ***, P < 0.001; **, P < 0.01; *, P < 0.05; n.s. P > 0.05.

Article Snippet: Endogenous peroxidases were blocked and antigens retrieved, and the slides were incubated overnight at 4°C with primary rabbit polyclonal anti-GALNT7 antibody (Atlas Antibodies Cat. #HPA064243, RRID: AB_2685223; 1:500).

Techniques: Expressing

Journal: Cancer Research Communications

Article Title: GALNT7 Stratifies dMMR/MSI Colorectal Cancer into Distinct Molecular Subsets Associated with Prognosis and PD-L1 Expression

doi: 10.1158/2767-9764.CRC-25-0270

Figure Lengend Snippet: IHC for GALNT7 expression in colorectal cancer. A–C, Representative images of a case of dMMR colorectal cancer with GALNT7-Low ( A ), showing tumor cell PD-L1 positive ( B ) and lack of Tn antigen expression ( C ). D–F, Representative images of a case of dMMR colorectal cancer with GALNT7-High ( D ), showing tumor cell PD-L1 negative ( E ) and strong Tn antigen expression ( F ). G, Proportion of GALNT7-Low and GALNT7-High in dMMR/MSI and pMMR/MSS colorectal cancers. H and I, Kaplan–Meier curves illustrating RFS according to GALNT7 expression by IHC in dMMR/MSI ( H ) and pMMR/MSS ( I ) colorectal cancers. The log-rank P value, Cox HR, and 95% CI are indicated. J, Relative proportion of tumor cell PD-L1 positive and negative in colorectal cancer according to GALNT7 IHC and MMR/MSI status. K, Tn antigen H-score in colorectal cancer according to GALNT7 IHC and MMR/MSI status. L–O, Levels of immune infiltration of CD8 + lymphocytes ( L ), CD4 + lymphocytes ( M ), Foxp3 + lymphocytes ( N ), and CD163 + macrophages ( O ) in colorectal cancer according to GALNT7 IHC and MMR/MSI status. ****, P < 0.0001; **, P < 0.01; *, P < 0.05; n.s. P > 0.05.

Article Snippet: Endogenous peroxidases were blocked and antigens retrieved, and the slides were incubated overnight at 4°C with primary rabbit polyclonal anti-GALNT7 antibody (Atlas Antibodies Cat. #HPA064243, RRID: AB_2685223; 1:500).

Techniques: Expressing

Journal: Cancer Research Communications

Article Title: GALNT7 Stratifies dMMR/MSI Colorectal Cancer into Distinct Molecular Subsets Associated with Prognosis and PD-L1 Expression

doi: 10.1158/2767-9764.CRC-25-0270

Figure Lengend Snippet: GALNT7 depletion and IFNγ-induced PD-L1 expression in MSI colorectal cancer cell lines. LS180, HCT116, LoVo, and RKO cells transfected with siRNAs targeting GALNT7 or scramble negative control in the presence or absence of IFNγ, analyzed using Western blotting.

Article Snippet: Endogenous peroxidases were blocked and antigens retrieved, and the slides were incubated overnight at 4°C with primary rabbit polyclonal anti-GALNT7 antibody (Atlas Antibodies Cat. #HPA064243, RRID: AB_2685223; 1:500).

Techniques: Expressing, Transfection, Negative Control, Western Blot